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Trypsin was neutralized with 500 l complete medium, the cells were resuspended and 100 l of the suspension was mixed with 6.9 ml Beckman Coulter Isoton II diluent (Fisher Scientific, NC2470899) for counting with a Beckman Coulter Z1 Coulter Cell Particle counter

Copper is a required cofactor for lysyl oxidase (connective-tissue crosslinking), for superoxide dismutase (antioxidant defense), and for enzymes that support angiogenesis, so a molecule that delivers copper in a controlled, cell-usable form has a coherent reason to influence tissue remodeling and vascular biology

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PubMed PMID: 24791752

Considering the majority of these procedures are unnecessarily being performed for degenerative disc disease alone, spine surgeons should be increasingly asked why they are offering these operations to their patients?(10) Surgeons at Leiden University Medical Centre in the Netherlands speculate that doctors go into a diagnosis of lumbar spinal stenosis with the thought that there is osteoarthritis a bony overgrowth on the spinal nerves